Correlations of quantitative MRI metrics with myelin basic protein (MBP) staining in a murine model of demyelination
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چکیده
منابع مشابه
Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
Aptamer ligands for myelin basic protein (MBP) were obtained using the systematic evolution of ligand by exponential enrichment (SELEX) method. Two clones were isolated from a pool of oligonucleotides and tested for MBP targeting. Using purified MBP, we demonstrated the binding activity of the aptamers and we also showed the affinity of MBP for oligonucleotides of specific length. Moreover, one...
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Source and Description of Probe. The probe was obtained from a pUC8 clone, pHBP-1, that carried the complete coding sequence of the human MBP gene (Kamholz et al., 1986; American Type Culture Collection, Rockville, MD). A 1.2-kb cDNA insert was excised from pHBP-1 using the enzyme EcoRI. Method of Detection. Sheep genomic DNA was extracted from whole blood. The DNA digests were resolved in 1% a...
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Background: Multiple sclerosis (MS), as a multifactorial autoimmune disease with complex genetic basis, causes demyelination in the central nervous system via cytokine responses to myelin antigens. Myelin basic protein (MBP) is the main protein component of the myelin sheath. HLA-DRB (human leukocyte antigen-DR beta) alleles, particularly HLA-DRB1*1501, may be of significance in the pathogenesi...
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Background: Progesterone as a sex steroid hormone is thought to affect and prevent demyelination, but its role in promoting myelin repair is far less investigated. In this study, remyelinating potential of progesterone in corpus callosum was evaluated on an experimental model of MS.Methods: In this experimental study, adult male C57BL/6 mice were fed with 0.2% (w/w) cuprizone in ground breeder ...
متن کاملAggregation of MBP in chronic demyelination
OBJECTIVES Misfolding of key disease proteins to an insoluble state is associated with most neurodegenerative conditions, such as prion, Parkinson, and Alzheimer's diseases. In this work, and by studying animal models of multiple sclerosis, we asked whether this is also the case for myelin basic protein (MBP) in the late and neurodegenerative phases of demyelinating diseases. METHODS To this ...
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ژورنال
عنوان ژورنال: NMR in Biomedicine
سال: 2019
ISSN: 0952-3480
DOI: 10.1002/nbm.4116